| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Institut National de la Santé et de la Recherche Médicale (INSERM) U425, Neuroimmunopharmacologie Pulmonaire, Faculté de Pharmacie, Université Louis Pasteur Strasbourg I, Illkirch Cedex, France. 2 Dept of Medicine, Division of Respiratory Medicine, Karolinska Hospital, 17176, Stockholm, Sweden
CORRESPONDENCE: N. Frossard, INSERM U425, Neuroimmunopharmacologie Pulmonaire, Faculté de Pharmacie, Université Louis Pasteur Strasbourg I, B.P. 24, 67 401, Illkirch Cedex, France. Fax: 33 390244309
Keywords: airway, glucocorticoid, inflammation, interleukin-1ß, nerve growth factor
Received: August 8, 2000
Accepted January 25, 2001
The project was funded by INSERM. C. Olgart was supported by the Swedish MRC, T. and R. Söderberg's Foundations, E. and E. Fernström's Foundation, and Comité Départemental du Bas-Rhin Contre les Maladies Respiratoires et la Tuberculose, UCB Institute of Allergy, Brussels, Belgium.
Nerve growth factor (NGF) has recently been suggested to contribute to inflammation and bronchial hyperresponsiveness in asthma. However, the cell types capable of NGF production in the human lung and airways, as well as the regulatory role of pro-inflammatory cytokines and of glucocorticoids on NGF secretion in pulmonary cells, have not been described.
Human pulmonary fibroblasts were cultured in the presence or absence of either interleukin-1ß (IL-1ß), tumour necrosis factor-
The human pulmonary fibroblasts constitutively secreted NGF in vitro. The rate of NGF secretion was shown to be cell density-dependent, since higher NGF secretion was detected in preconfluent cells, i.e. ones with less established cell-to-cell contact (41.0±5.0 pg·10–6 cells at 80% confluence), than cells in higher densities (8.2±3.4 pg·10–6 cells at 100% confluence). Stimulation with the pro-inflammatory cytokines IL-1ß (0.3–30 U·mL–1) or TNF-
In conclusion, human lung fibroblasts may serve as a source of nerve growth factor in the lung, positively regulated by the asthma-associated and pro-inflammatory cytokines interleukin-1ß and tumour necrosis factor-
(TNF-) and/or glucocorticoids. NGF secretion was measured by enzyme-linked immunosorbent assay. (0.1–30 ng·mL–1) dose- and time-dependently (8–72 h) elevated the NGF secretion (effective concentration causing 50% of the maximum response (EC50)=2.9 U·mL–1 and 1.0 ng·mL–1, respectively). Treatment with the glucocorticoid budesonide (10–7 M) markedly reduced the constitutive secretion of NGF by 42%, and attenuated the cytokine-stimulated NGF secretion to the same level. , and negatively regulated by the anti-inflammatory glucocorticoids.
This article has been cited by other articles:
|
|
 |
|
  L. Jornot, J. S. Lacroix, and T. Rochat Neuroendocrine cells of nasal mucosa are a cellular source of brain-derived neurotrophic factor Eur. Respir. J., September 1, 2008; 32(3): 769 - 774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Frossard, E. Naline, C. Olgart Hoglund, O. Georges, and C. Advenier Nerve growth factor is released by IL-1{beta} and induces hyperresponsiveness of the human isolated bronchus Eur. Respir. J., July 1, 2005; 26(1): 15 - 20. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Ito, A. Kanehiro, K. Matsumoto, A. Hirano, K. Ono, H. Maruyama, M. Kataoka, T. Nakamura, E. W. Gelfand, and M. Tanimoto Hepatocyte Growth Factor Attenuates Airway Hyperresponsiveness, Inflammation, and Remodeling Am. J. Respir. Cell Mol. Biol., April 1, 2005; 32(4): 268 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ricci, L. Felici, S. Mariotta, F. Mannino, G. Schmid, C. Terzano, G. Cardillo, F. Amenta, and E. Bronzetti Neurotrophin and Neurotrophin Receptor Protein Expression in the Human Lung Am. J. Respir. Cell Mol. Biol., January 1, 2004; 30(1): 12 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Olgart Hoglund, F. de Blay, J-P. Oster, C. Duvernelle, O. Kassel, G. Pauli, and N. Frossard Nerve growth factor levels and localisation in human asthmatic bronchi Eur. Respir. J., November 1, 2002; 20(5): 1110 - 1116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Fryer and D. B. Jacoby Plasticity of cholinergic and tachykinergic nerves: the convergence of the twain Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L907 - L908. [Full Text] [PDF]
|
|
|
|
 |
|
 G. Path, A. Braun, N. Meents, S. Kerzel, D. Quarcoo, U. Raap, G. W. Hoyle, W. A. Nockher, and H. Renz Augmentation of Allergic Early-Phase Reaction by Nerve Growth Factor Am. J. Respir. Crit. Care Med., September 15, 2002; 166(6): 818 - 826. [Abstract] [Full Text]
|
|
|
|
|
|
V. Freund, F. Pons, V. Joly, E. Mathieu, N. Martinet, and N. Frossard Upregulation of nerve growth factor expression by human airway smooth muscle cells in inflammatory conditions Eur. Respir. J., August 1, 2002; 20(2): 458 - 463. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
