Chemokine involvement in tetracycline-induced pleuritis

Sclerosants such as tetracycline (TCN) have often been used in the control of malignant pleural effusions. Although the resultant inflammatory response is probably important in the ensuing pleural fibrosis, the signals responsible for the cellular influx into the pleural space following TCN instillation are not well understood. This study, therefore, sought to determine whether the chemokines interleukin-8 (IL-8), growth-related protein (Gro), and monocyte chemotactic protein-1 (MCP-1) were locally elaborated within the first 72 h following intrapleural TCN administration. TCN induced an exudative effusion with high lactate dehydrogenase activity. Although there was no significant change in the pleural fluid total leukocyte content, the median polymorphonuclear neutrophil concentration decreased from 1.067x10(6) to 2.03x10(5) cells x mL(-1) between 24 and 72 h, whereas the median macrophage concentration increased from 1.44x10(5) to 5.98x10(5) cells x mL(-1) over the same period. Furthermore, IL-8, Gro and MCP-1 concentrations decreased between 24 and 72 h. Immunocytochemistry indicated expression of IL-8 by pleural mesothelial cells 24 h, but not 72 h, following TCN administration. The data suggest that local elaboration of interleukin-8 and growth-related protein, in part of mesothelial origin, may influence neutrophil recruitment in tetracycline-induced pleuritis.

This article has been cited by other articles:

				S. Idell, T. Allen, S. Chen, K. Koenig, A. Mazar, and A. Azghani Intrapleural activation, processing, efficacy, and duration of protection of single-chain urokinase in evolving tetracycline-induced pleural injury in rabbits Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L25 - L32. [Abstract] [Full Text] [PDF]

				Y. B. Guo, I. Kalomenidis, M. Hawthorne, K. S. Parman, K. B. Lane, and R. W. Light Pleurodesis Is Inhibited by Anti-Vascular Endothelial Growth Factor Antibody Chest, September 1, 2005; 128(3): 1790 - 1797. [Abstract] [Full Text] [PDF]

				Y.C.G. Lee, K.B. Lane, O. Zoia, P.J. Thompson, R.W. Light, and T.S. Blackwell Transforming growth factor-{beta} induces collagen synthesis without inducing IL-8 production in mesothelial cells Eur. Respir. J., August 1, 2003; 22(2): 197 - 202. [Abstract] [Full Text] [PDF]

				S. N. Georas, L. A. Beck, and C. Stellato What Is Eotaxin Doing in the Pleura? . Insights into Innate Immunity from Pleural Mesothelial Cells Am. J. Respir. Cell Mol. Biol., April 1, 2002; 26(4): 384 - 387. [Full Text] [PDF]

				N. Hata, K. Tanaka, T. Imaizumi, T. Ohara, T. Ohba, T. Shinada, and T. Takano Clinical Significance of Pleural Effusion in Acute Aortic Dissection Chest, March 1, 2002; 121(3): 825 - 830. [Abstract] [Full Text] [PDF]